Donor site morbidity and the limited availability of donor tissue are major drawbacks for autologous grafting, while allogeneic transplants pose major risks from graft-versus-host reactions and from life-long immunosuppression. However, harvesting tissue for grafting creates a donor site wound. When biological tissue is damaged by disease or trauma beyond its capacity to regenerate, replacing the defective tissue by transplantation is often the only curative option. Our method led to faster and more orderly re-epithelialization but unexpectedly did not improve the retention of dermal architecture, which reveals a hitherto unappreciated role for tissue morphology in determining dermal remodeling outcomes. In this study, we developed a magnetically induced assembly method to produce constructs of densely packed, oriented MSTCs that closely resemble the overall architecture of full-thickness skin to test the hypothesis that maintaining MSTCs’ orientation could further hasten healing and restore a normal dermis. However, despite MSTCs containing all the cellular and extracellular contents of full-thickness skin, normal dermal architecture was not restored by random MSTCs. We recently found that full-thickness micro skin tissue columns (MSTCs) can be harvested with minimal donor site morbidity, and that MSTCs applied to wounds “randomly” (without maintaining their natural epidermal-dermal orientation) can accelerate re-epithelialization. Skin wounds are immense medical and socioeconomic burdens, and autologous skin grafting remains the gold standard for wound repair.
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